Microtumor Model Construction and Drug Sensitivity Detection Technology Chinese Expert Consensus (2023 Edition) 公告 2024-12-16 08:51:00 Written By Kym Kilbourne Title: Microtumor Model Construction and Drug Sensitivity Detection Technology Chinese Expert Consensus (2023 Edition) Abstract The "Microtumor Model Construction and Drug Sensitivity Detection Technology Chinese Expert Consensus (2023 Edition)" was organized and written by the Organoid and Organ-on-a-Chip Branch of the Chinese Society of Biomedical Engineering, aiming to standardize and guide the construction of microtumor (microtumor Patient-Derived Tumor, PTC) models and their application in drug sensitivity detection. Microtumor PTC is a new type of primary 3D tumor model that maintains a high degree of similarity with the original tumor tissue in pathological histological characteristics and molecular genetic characteristics, and can simulate the microenvironmental characteristics of the original tumor tissue. Overview of Microtumor PTC Models Microtumor PTC models are formed by treating fresh tumor samples from patients with single-cellization ex vivo, and then suspending and culturing in low-adhesion culture dishes with specialized media to form 3D tumor spheroids. This model retains fibroblasts and immune cells from the original tumor tissue during the culture process, highly replicating the microenvironmental characteristics of the original tumor tissue. Features of Microtumor PTC Models Microtumor PTC models are characterized by low sample requirement, short culture cycle, and high culture success rate. They can truly simulate the biological characteristics of tumors in patients' bodies in vitro, including pathological histological characteristics, molecular genetic characteristics, and to some extent, simulate tumor microenvironmental characteristics. Quality Control of Microtumor PTC Models The consensus emphasizes the importance of quality control in the collection, preservation, and transportation processes of tumor tissue for the culture of microtumor PTC models and proposes specific operational standards and precautions to ensure the success and reliability of the model culture. Drug Sensitivity Detection Based on Microtumor PTC Due to its high tumor fidelity, the microtumor PTC model is recommended for the detection of antitumor drug sensitivity. This detection technology can predict the efficacy of chemotherapy drugs and some targeted drugs, providing a basis for the development of individualized and precise clinical medication plans. Key Operational Points of Microtumor PTC Drug Sensitivity Detection Technology The consensus provides detailed operational points for conducting drug sensitivity detection based on the microtumor PTC model, including the number of spheroids in the experimental wells, the setting of replicate wells, and the use of artificial intelligence cell image recognition systems to determine results. Application Prospects of Microtumor PTC Models and Drug Sensitivity Detection Technology The application of microtumor PTC models and their drug sensitivity detection technology is accelerating in the fields of breast cancer, lung cancer, and digestive system tumors. With the continuous optimization of technology, it is expected to provide scientific research and individualized precision treatment support for more solid tumors and some rare types of tumors. Conclusion As a new type of in vitro tumor model, microtumor PTC models have a broad application prospect in the fields of basic tumor research, precision treatment, and new drug development. This consensus will be gradually improved and revised with the innovation of microtumor PTC technology to promote its application and development in the clinic. Kym Kilbourne
Microtumor Model Construction and Drug Sensitivity Detection Technology Chinese Expert Consensus (2023 Edition) 公告 2024-12-16 08:51:00 Written By Kym Kilbourne Title: Microtumor Model Construction and Drug Sensitivity Detection Technology Chinese Expert Consensus (2023 Edition) Abstract The "Microtumor Model Construction and Drug Sensitivity Detection Technology Chinese Expert Consensus (2023 Edition)" was organized and written by the Organoid and Organ-on-a-Chip Branch of the Chinese Society of Biomedical Engineering, aiming to standardize and guide the construction of microtumor (microtumor Patient-Derived Tumor, PTC) models and their application in drug sensitivity detection. Microtumor PTC is a new type of primary 3D tumor model that maintains a high degree of similarity with the original tumor tissue in pathological histological characteristics and molecular genetic characteristics, and can simulate the microenvironmental characteristics of the original tumor tissue. Overview of Microtumor PTC Models Microtumor PTC models are formed by treating fresh tumor samples from patients with single-cellization ex vivo, and then suspending and culturing in low-adhesion culture dishes with specialized media to form 3D tumor spheroids. This model retains fibroblasts and immune cells from the original tumor tissue during the culture process, highly replicating the microenvironmental characteristics of the original tumor tissue. Features of Microtumor PTC Models Microtumor PTC models are characterized by low sample requirement, short culture cycle, and high culture success rate. They can truly simulate the biological characteristics of tumors in patients' bodies in vitro, including pathological histological characteristics, molecular genetic characteristics, and to some extent, simulate tumor microenvironmental characteristics. Quality Control of Microtumor PTC Models The consensus emphasizes the importance of quality control in the collection, preservation, and transportation processes of tumor tissue for the culture of microtumor PTC models and proposes specific operational standards and precautions to ensure the success and reliability of the model culture. Drug Sensitivity Detection Based on Microtumor PTC Due to its high tumor fidelity, the microtumor PTC model is recommended for the detection of antitumor drug sensitivity. This detection technology can predict the efficacy of chemotherapy drugs and some targeted drugs, providing a basis for the development of individualized and precise clinical medication plans. Key Operational Points of Microtumor PTC Drug Sensitivity Detection Technology The consensus provides detailed operational points for conducting drug sensitivity detection based on the microtumor PTC model, including the number of spheroids in the experimental wells, the setting of replicate wells, and the use of artificial intelligence cell image recognition systems to determine results. Application Prospects of Microtumor PTC Models and Drug Sensitivity Detection Technology The application of microtumor PTC models and their drug sensitivity detection technology is accelerating in the fields of breast cancer, lung cancer, and digestive system tumors. With the continuous optimization of technology, it is expected to provide scientific research and individualized precision treatment support for more solid tumors and some rare types of tumors. Conclusion As a new type of in vitro tumor model, microtumor PTC models have a broad application prospect in the fields of basic tumor research, precision treatment, and new drug development. This consensus will be gradually improved and revised with the innovation of microtumor PTC technology to promote its application and development in the clinic. Kym Kilbourne
